圖1.用抗BCAS2抗體和抗PLZF抗體對(duì)小鼠P8睪丸的石蠟切片進(jìn)行染色。用Hoechst 33342對(duì)DNA進(jìn)行染色,并用Fluoromount-G®(貨號(hào)0100-01)封片。圖片來(lái)自Liu W , Wang F , Xu Q, Shi J , Zhang X , Liu X , et al . BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017; 8: 14182.
圖2. 用AF488標(biāo)記的鬼筆環(huán)肽對(duì)卷身羅斯考夫蠕蟲胚胎全標(biāo)本進(jìn)行染色,并用Fluoromount-G®(貨號(hào)0100-01)封片。圖片來(lái)自Semmler H, Bailly X, Wanninger A. Myogenesis in the basal bilaterian Symsagittifera roscoffensis (Acoela). Front Zool.2008; 5:14.
圖3. 用anti-Zpr3抗體對(duì)冰凍斑馬魚視網(wǎng)膜切片進(jìn)行染色,然后用AF543二抗和MBODEC染色并用Fluoromount-G®(貨號(hào)0100-01)封片。圖片來(lái)自Watanabe K ,Nishimura Y, Oka T, Nomoto T, Kon T, Shitou T,et al. In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neurosci.2010; 11:116.
圖4.HCC827細(xì)胞先用抗E-鈣粘蛋白抗體染色,隨后用二抗染色,最后用DAPI Fluoromount-G®(貨號(hào)0100-20)封片。圖片來(lái)自Narita M, Shimura E, Nagasawa A , Aiuchi T , Suda Y ,Hamada Y et al . Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLOS One.2017; 12 (2): e0172115.
圖5.在無(wú)血清培養(yǎng)基中生長(zhǎng)的人中性粒細(xì)胞用抗中性粒細(xì)胞彈性蛋白酶抗體染色,然后用AF555偶聯(lián)的二抗染色,并用DAPI Fluoromount-G®(貨號(hào)0100-20)封片。圖片來(lái)自Kamoshida G, Kikuchi-Ueda T, Nishida S, Tansho-Nagakawa S , Kikuchi H, Ubagai T , et al . Spontaneous formation of neutrophil extracellular traps in serum-free culture conditions. FEBS Open Bio. 2017; 7: 877-86.