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    1. 技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > Enzo Life Sciences:GFP-CERTIFIED®FLUOFORTE®鈣離子檢測試劑盒

      Enzo Life Sciences:GFP-CERTIFIED®FLUOFORTE®鈣離子檢測試劑盒

      更新時間:2023-11-16   點擊次數(shù):492次

      Enzo Life SciencesGFP-CERTIFIED® FLUOFORTE® Calcium assay kit提供了一種熒光分析方法,用于檢測廣泛生物靶標的細胞內(nèi)鈣動員情況。相對于其他商業(yè)化的染料,GFP-CERTIFIED® FLUOFORTE®染料是最亮和最-靈-敏的熒光鈣指示劑該試劑盒為貼壁和非貼壁細胞提供了更少步驟,更低變異性,更簡易鈣檢測。此外,它既不需要洗滌步驟,也不需要添加外源性淬滅染料,避免受體-配體相互作用動力學的干擾。

      Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit可用于細胞內(nèi)鈣離子的高通量檢測,鈣離子是重要的第二信使,常用于初步篩選中監(jiān)測G蛋白偶聯(lián)受體激活。

       

      產(chǎn)品特色

      ● 可監(jiān)測表達GFP細胞中的鈣離子動員情況

      ● 最明亮、最-靈-敏的熒光Ca2+指示劑

      ● 獲得的 EC50和IC50值與使用鈣離子熒光探針 Rhod-4 AM和Fluo-4 AM 相當

      ● 更大范圍的檢測窗口

      ● 使用方便,無需洗滌,無需淬滅染料

       

      實驗示例

      GFP-CERTIFIED®FLUOFORTE®鈣離子檢測試劑盒——ENZO熱銷產(chǎn)品

       

      GFP-CERTIFIED®FLUOFORTE®鈣離子檢測試劑盒——ENZO熱銷產(chǎn)品 

      2. U-2 OS細胞分別與(AGFP-CERTIFIED® FLUOFORTE®AM染料BRhod-2 AM染料孵育。洗滌細胞并用終濃度為100nMATP處理,并立即成像。GFP-CERTIFIED® FLUOFORTE® AM染料比Rhod-2 AM表現(xiàn)出更亮的熒光強度。

       

      產(chǎn)品信息

      產(chǎn)品貨號

      ENZ-51020-KP010/ ENZ-51020-KP100

      產(chǎn)品名稱

      GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

      規(guī)       格

      10*96wells/100*96wells

      短期保存

      -20°C

      長期保存

      -80°C

      試劑盒組分

      Starter pack (Prod. No. ENZ-51020-KP010):

      Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial

      Reagent B (dye efflux inhibitor), 10ml

      Reagent C (Hanks’ buffer with 20mM HEPES, 100ml)

      Sufficient for 10 x 96-well plates


      High-throughput (Prod. No. ENZ-51020-KP100):

      Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials

      Reagent B (dye efflux inhibitor), 10 x 10ml

      Sufficient for 100 x 96-well plates

       

      部分產(chǎn)品引用文獻

      1. Perfluorooctane sulfonate and bisphenol A induce a similar level of mast cell activation via a common signaling pathway, Fyn-Lyn-Syk activation: S.J. Park, et al.; Food Chem. Toxicol. 156, 112478 (2021)

      2. horbol myristate acetate induces differentiation of THP-1 cells in a nitric oxide-dependent manner: Y.Y. Chang, et al.; Nitric Oxide 109, 33 (2021)

      3. Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation: C. Tr?tschel, et al.; EMBO J. 39, e102723 (2020)

      4. Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium: J.N. Hansen, et al.; Elife 9, e57907 (2020)

      5. Interplay between sub-cellular alterations of calcium release and T-tubular defects in cardiac diseases: M. Scardigli, et al.; Front. Physiol. 9, 1474 (2018)

      6. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype: H. Vienken, et al.; Sci. Rep. 7, 43575 (2017)

      7. Novel insights on the relationship between T-tubular defects and contractile dysfunction in a mouse model of hypertrophic cardiomyopathy: C. Crocini, et al.; J. Mol. Cell. Cardiol. 91, 42 (2016)

      8. Sulfur dioxide protects against collagen accumulation in pulmonary artery in association with downregulation of the transforming growth factor β1/smad pathway in pulmonary hypertensive rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016)

      9. T-tubular electrical defects contribute to blunted β-adrenergic response in heart failure: C. Crocini, et al.; Int. J. Mol. Sci. 17, 1471 (2016)

      10. Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)

       


      Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag

      詳情請聯(lián)系Enzo Life Sciences金牌代理——欣博盛生物