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      Epicypher欣博盛生物新品 | 核酸提取緩沖液
      
        
      更新時間：2023-12-08      點擊次數(shù)：511次
      
        
      Epicypher新品 | 核酸提取緩沖液

      CUTANA™ 細胞核提取緩沖液是CUT&RUN和CUT&Tag實驗中，用于從培養(yǎng)的細胞和組織中提取細胞核的必需試劑。這種緩沖液可與多種真核細胞和組織兼容。以下是使用該緩沖液成功進行細胞核分離的細胞和組織類型：
      mouse NIH3T3 fibroblast cells
      human A549 non-small cell lung cancer (NSCLC) cells
      human K562 leukemia cells
      human NCI-H1299 non-small cell lung cancer (NSCLS cells)
      human bone marrow derived macrophages
      human TIG-1 fetal lung cells
      human monocyte derived macrophages
      human LoVo colorectal cancer cells
      human MV-4-11 macrophage cells
      human LNCaP prostate carcinoma cells
      human SUM149 triple negative breast cancer (TNBC) cells
      human renal primary cells
      human GM24385 B-lymphocyte (aka HG002) cells
      human peripheral blood mononuclear cells (PBMCs)
      human MCF7 breast cancer cells
      human intestinal tissue
      human MDA-MB-231 breast cancer cells
      human HEPM embryonic cells
      human SK-MEL-2 melanoma cells

       
      只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA™ 細胞核提取緩沖液針對CUT&RUN和CUT&Tag實驗流程進行了優(yōu)化，從而獲得高質(zhì)量的細胞核，助力您的基因組圖譜分析。
       
      試劑盒組成
      組分
      貨號
      CUTANA™ Pre-Nuclei Extraction Buffer      
      21-1026a     
      1 M Spermidine
      21-1026b

      保存條件
      將Pre-Nuclei Extraction Buffer 4oC儲存, 1 M亞精胺-20oC儲存。
      自收到之日起可穩(wěn)定儲存6個月。

      驗證數(shù)據(jù)
      Epicypher新品 | 核酸提取緩沖液
      Figure 1: Nuclei Extraction
      Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.

      訂購詳情
      貨號
      產(chǎn)品名稱
      規(guī)格
      21-1026
      CUTANA™ Nuclei Extraction Buffer
      100 Reactions
       


      Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag
      如需了解更多詳細信息或相關產(chǎn)品，請聯(lián)系EpiCypher中國授權代理商-欣博盛生物