ENZO熱銷產(chǎn)品——FLUOR DE LYS® HDAC fluorometric activity assay kit組蛋白去乙?；笩晒饣钚詸z測試劑盒
目前，組蛋白去乙?；敢种苿┮呀?jīng)顯示出其作為抗腫瘤藥物的前景，這也自然激發(fā)了研究者們對于篩選HDAC（Histone deacetylase，組蛋白去乙酰化酶）抑制化合物的興趣。Enzo的FLUOR DE LYS®HDAC熒光活性檢測試劑盒（貨號：BML-AK500-0001）是一種靈敏、便捷的組蛋白去乙?；笝z測試劑盒，可替代使用傳統(tǒng)的放射性標(biāo)記的乙?；M蛋白和肽/HPLC法檢測組蛋白去乙?；傅姆桨浮?/span>
FLUOR DE LYS®去乙?；笝z測平臺是Enzo的升級酶活性檢測系列產(chǎn)品的基礎(chǔ)，其中的產(chǎn)品就包括用于篩選HDAC（組蛋白去乙?；福┗钚哉{(diào)節(jié)劑和Sirtuin（長壽蛋白，一種去乙?；福┗钚哉{(diào)節(jié)劑的試劑盒。Enzo還可提供基于熒光共振能量轉(zhuǎn)移（FRET）的試劑盒，用以檢測基質(zhì)金屬蛋白酶（MMPs）和天冬氨酸特異性半胱氨酸蛋白酶（Caspase）的活性，以及提供基于磷酸特異性抗體的激酶檢測試劑盒。
FLUOR DE LYS® HDAC fluorometric activity assay kit基于Enzo特-有的 FLUOR DE LYS®（熒光法組蛋白去乙?；?賴氨酸）底物和顯影劑組合，只需2步簡單的混合步驟，即可在同一個(gè)96 孔板上進(jìn)行檢測。首先，將含有乙?；嚢彼醾?cè)鏈的FLUOR DE LYS®底物與含有HDAC活性的樣品（HeLa核或其他提取物、純化酶、珠結(jié)合免疫復(fù)合物等）一起孵育。底物的去乙?；瘯?huì)使底物增敏，所以在第二步中，將其與FLUOR DE LYS®顯影劑混合后會(huì)產(chǎn)生熒光團(tuán)。
該檢測方法目前已經(jīng)成功用于所有已知的I類HDAC——HDAC1、HDAC2、HDAC3和HDAC8（見產(chǎn)品數(shù)據(jù)表）和II 類HDAC 4-7、9和10，以及人類Sir2同源物和SIRT1（見產(chǎn)品數(shù)據(jù)表）的制備。Enzo Life Sciences的研究表明，F(xiàn)LUOR DE LYS®底物具有細(xì)胞滲透性，并可在原位被細(xì)胞HDAC去乙酰化。脫乙酰化的底物在細(xì)胞內(nèi)積聚后，可通過向細(xì)胞裂解液中加入FLUOR DE LYS®顯影劑進(jìn)行定量測定。
FLUOR DE LYS® HDAC fluorometric activity assay kit
規(guī)格
1*96wells
使用/穩(wěn)定性
Store all components, except the microtiter plate, at -80℃ for the highest stability. The HeLa Nuclear Extract, BML-KI140, must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused extract should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the extract into separate tubes and store at -80℃. The FLUOR DE LYS® Substrate, BML-KI104, when diluted in Assay Buffer, may precipitate after freezing and thawing. It is best, therefore, to dilute only the amount needed to perform the assays of that day.
運(yùn)輸
Dry Ice
長期保存
-80℃
試劑盒組分
Nuclear Extract from HeLa Cells (human cervical cancer cell line) (Prod. No. BML-KI140-0100) (100 µl; In 0.1M KCl, 20mM HEPES/NaOH, pH 7.9, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5Mm PMSF, prepared according to a modification of J.D. Dignam et al. (1983) and S.M. Abmayr et al. (1988)). Storage: -80℃, avoid freeze/thaw cycles FLUOR DE LYS® Substrate (Prod. No. BML-KI104-0050) (50 µl; 50mM in DMSO) Storage: -80℃ FLUOR DE LYS® Developer Concentrate (20×) (Prod. No. BML-KI105-0300) (300 µl; 20×stock solution, dilute in assay buffer before use) Storage: -80℃ Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR309-9090) (100 µl; 0.2mM in DMSO) Storage: -80℃ FLUOR DE LYS® Deacetylated Standard (Prod. No. BML-KI142-0030) (30 µl; 10mM in DMSO) Storage: -80℃ HDAC Assay Buffer (Prod. No. BML-KI143-0020) (20 ml; 50mM TRIS/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2) Storage: -20℃ 1/2 volume microplate (Prod. No. BML-KI101) Storage: Ambient 1/2 volume white microplate (Prod. No. BML-K571) Storage: Ambient
產(chǎn)品引用文獻(xiàn)：
The activation of histone deacetylases 4 prevented endothelial dysfunction: A crucial mechanism of HuangqiGuizhiWuwu Decoction in improving microcirculation dysfunction in diabetes: M. Chen, et al.; J. Ethnopharmacol. 307, 116240 (2023), Abstract;
Four New Anthraquinones with Histone Deacetylase Inhibitory Activity from Ventilago denticulata Roots: N. Hangsamai, et al.; Molecules 27, 1088 (2022), Abstract;
研究相關(guān)的產(chǎn)品推薦： FLUOR DE LYS® HDAC Fluorometric Activity Assay Kit FLUOR DE LYS® HDAC6 Fluorometric Drug Discovery Kit FLUOR DE LYS® SIRT1 Fluorometric Drug Discovery Assay Kit Matrix Metalloproteinase (MMP) Multipack-1 MMP Inhibitor Profiling Kit, Fluorometric Matrix Metalloproteinase-2 (MMP-2) Fluorometric Drug Discovery Kit, RED Akt Kinase Activity Kit PKA Kinase Activity Kit PKC Kinase Activity Kit Nitric Oxide (NO2-/NO3-) Detection Kit
在線咨詢
電話咨詢
