Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are……
Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
Product Details
Alternative Name:
Cyanine 3-uridine-5’-triphosphate (enhanced)
Concentration:
10mM
Formulation:
Liquid. Solution in water.
Excitation maximum:
550 nm
Emission maximum:
564 nm
Extinction Coefficient:
150,000 M-1 cm-1 (550 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Purity:
≥93% (HPLC)
Purity Detail:
Purified by ion-exchange chromatography.
Appearance:
Dark pink liquid.
Shipping:
Shipped on Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
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Product Literature References
Borna disease virus phosphoprotein triggers the organization of viral inclusion bodies by liquid-liquid phase separation: Y. Hirai, et al.; Int. J. Biol. Macromol. 192, 55 (2021), Abstract;
NORAD-induced Pumilio phase separation is required for genome stability: M.M. Elguindy, et al.; Nature (2021), Abstract;
Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression: H. Kim, et al. ; Sci. Rep. 5, 12737 (2015), Application(s): Labeling of mRNA-NPs, Abstract;
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