久久午夜影院,国产大鸡巴九色,丰满人妻一区=区三区在线视频53,五月天婷婷激情四射在线观看

    <source id="7npa8"><tr id="7npa8"><dfn id="7npa8"></dfn></tr></source>
    1. 產(chǎn)品中心您現(xiàn)在的位置:首頁 > 產(chǎn)品展示 > > 細(xì)胞培養(yǎng) > Primocin™

      Primocin™

      更新時間:2022-01-28

      簡要描述:

      Primocin&#174; is designed to offer complete protection of primary cells from microbial contamination. Primocin&#174; is a broad-spectrum antibiotic formulation that is gentle on your cells but lethal to the ……

      型號:廠商性質(zhì):代理商瀏覽量:953

      Antimicrobial agent for primary cells

      Primocin® is designed to offer complete protection of primary cells from microbial contamination. Primocin® is a broad-spectrum antibiotic formulation that is gentle on your cells but lethal to the microbes. There are a number of common sources of microbial contamination especially in the isolation of primary cells from both animal and human tissue, including commensal flora and/or subclinical infections.

      Use of Primocin® in primary cell cultures
      Use of Primocin® in primary cell cultures

      Key Features:

      • Active against bacteria, mycoplasma, and fungi

      • A common addition to various standardized primary cell culture protocols

      • Used throughout culturing process (e.g. culture media, wash solutions, and  biopsy storage media) 

      • Highly cited in the literature

       

      Primocin® is composed of four compounds, of which three, act directly on Gram-positive bacteria, Gram-negative bacteria, and mycoplasmas. The primary mode of action for these compounds is to block microbial DNA and protein synthesis. The fourth compound in Primocin® eradicates fungi, including yeasts, by directly targeting processes at the fungal membrane.

       

      Primocin® is successfully used in many types of primary cell cultures (see citations) including:

      • Differentiated mouse- and human-derived cells such as astrocytes and immune cells (e.g. natural killer cells)

      • Stem cells such as mouse- and human-derived embryonic and pluripotent stem cells

      • 3D cellular models (i.e. organoid and spheroids) such as colon epithelial and carcinoma organoids

       

      Primocin® is made in-house at InvivoGen and is of the highest quality with rigorous quality control performed to ensure the absence of lot-to-lot variation.


      Specifications

      Product concentration: 50 mg/ml

      Recommended working concentration: 100 μg/ml

      • 1 ml vial of Primocin® is sufficient for 500 ml of culture.

      • 20 ml bottle of Primocin® is sufficient for 10 liters of culture.

      Quality Control:

      • Physicochemical characterization (pH and appearance) has been confirmed

      • Endotoxin levels: < 0.5 EU/mg

      • Potency has been validated on bacterial and fungal reference strains


      Contents

      Primocin® is provided as a cell culture tested, sterile filtered, light yellow solution at a concentration of 50 mg/ml.

      This product is available in three pack sizes:

      • ant-pm-05 - 5 x 1 ml vial (250 mg) 

      • ant-pm-1 - 10 x 1 ml vial (500 mg)

      • ant-pm-2 - 1 x 20 ml bottle (1 g)

       Primocin® is shipped at room temperature.
       Upon receipt Primocin® can be stored at 4°C for 3 months or at -20°C for long term storage


      Details

      Use of Primocin® in primary cell cultures

      Primocin® is frequently cited in the literature for use in the protection of a number of different primary cell cultures. This includes (but is not limited to):

      • Numerous human and murine primary cell cultures, including fibroblasts [1], glial cells [2], astrocytes [3], and immune cells (e.g. NK cells) [4].

      • Culturing and reprogramming of human and murine embryonic cells [5] and pluripotent stem cells [6].

      • 3D cell cultures including colon epithelial and carcinoma organoids [7] as well as breast, bladder, and prostate cancer organoids [8-10].

      • A number of published standardized protocols such as tumor organoid-T-cell co-culture systems [11].


      References:

      1. Ferrer-Mayorga, G. et al., 2019. Vitamin D and Wnt3A have additive and partially overlapping modulatory effects on gene expression and phenotype in human colon fibroblasts. Sci Rep 9:8085.
      2. Bussian T.J. et al., 2018. Clearance of senescent glial cells prevents tau-dependent pathology and cognitive decline. Nature. 562:578?82.
      3. Grabner, G.F. et al., 2016. Deletion of Monoglyceride Lipase in Astrocytes Attenuates Lipopolysaccharide-induced Neuroinflammation. J Biol Chem 291;913?23.
      4. Garcia-Beltran, W.F. et al., 2016. Open conformers of HLA-F are high-affinity ligands of the activating NK-cell receptor KIR3DS1. Nat Immunol 17:1067-74.
      5. Kogata N. et al., 2018. Sox9 regulates cell state and activity of embryonic mouse mammary progenitor cells. Commun Biol. 1:228.
      6. Park S. et al., 2018. Generation of human-induced pluripotent stem cells using a defined, feeder-free reprogramming system. Curr Protoc Stem Cell Biol 45.
      7. Urbischek M. et al., 2019. Organoid culture media formulated with growth factors of defined cellular activity. Sci Rep 9:6193.
      8. Sachs N. et al., 2018. A living biobank of breast cancer organoids captures disease heterogeneity. Cell. 172:373-86.
      9. Lee S.H. et al., 2018. Tumor evolution and drug response in patient-derived organoid models of bladder cancer. Cell 173:515-28.
      10. Xu H. et al., 2018. Organoid technology and applications in cancer research. J Hematol Oncol 11:116.
      11. Cattaneo, C.M. et al., 2020. Tumor organoid–T-cell coculture systems. Nat Prot 15, 15-39.


      如需購買或咨詢該產(chǎn)品技術(shù)問題,請聯(lián)系Invivogen一級代理商-欣博盛生物

      留言框

      • 產(chǎn)品:

      • 您的單位:

      • 您的姓名:

      • 聯(lián)系電話:

      • 常用郵箱:

      • 省份:

      • 詳細(xì)地址:

      • 補(bǔ)充說明:

      • 驗(yàn)證碼:

        請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7